Journal: Progress in Biomaterials

Article Title: Nanohydroxyapatite incorporated photocrosslinked gelatin methacryloyl/poly(ethylene glycol)diacrylate hydrogel for bone tissue engineering

doi: 10.1007/s40204-021-00150-x

Figure Lengend Snippet: Physical and biological properties of GMP/GMPH hydrogels. a Equilibrium swelling ratio (ESR) of GMP and GMPH hydrogels (n = 6, unpaired two-tailed t test, P = 0.0195, *P < 0.05); b Equilibrium water content (EWC) of GMP and GMPH hydrogels (n = 6, unpaired two-tailed t test, P = 0.0190, *P < 0.05); c Gelation ratio of GMP and GMPH hydrogels (n = 6, unpaired two-tailed t test, P = 0.0114, *P < 0.05); d Biodegradation assay showed an accelerated degradation rate (%) of GMP hydrogel in week 2 (n = 6, unpaired two-tailed t test, P = 0.0015, *P < 0.05). GMPH hydrogel showed a slower degradation rate than GMP hydrogel in week 2 (n = 6, unpaired two-tailed t test, P = 0.0004, *P < 0.05); e MTT assay showing the viability of MG63 cells treated with the extracts of hydrogels. All error bars represent mean ± s.e.m. GM (GelMA), GMP (GelMA/PEGDA), GMPH (GelMA/PEGDA-nanohydroxyapatite)

Article Snippet: The degradation rate (DR) of the hydrogel scaffolds was obtained from the following calculation: DR % = W 0 - W f W 0 ∗ 100 5 Cell culture MG63 osteosarcoma cell line was procured from the National Centre for Cell Science, Pune, India.

Techniques: Two Tailed Test, MTT Assay

Journal: Marine Drugs

Article Title: Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization

doi: 10.3390/md19040194

Figure Lengend Snippet: Confocal laser scanning microscopy for: MSC/OEC co-cultures on day 7 for control ( a ) and 10 µg/mL treatment of Fvc ( b ), Fs1 ( c ), and Fs2 ( d ); MG63/OEC co-cultures on day 7 for control ( e ) and 10 µg/mL treatment of Fvc ( f ), Fs1 ( g ), and Fs2 ( h ). Endothelial marker VE-cadherin is depicted in green, and nuclei are depicted in blue. The scale bar represents 100 μm.

Article Snippet: MSC or the osteosarcoma cell line MG63 (ATCC, Wesel, Germany) were seeded at a density of 40.000 cells/cm 2 on collagen I (Corning, Badford, MA, USA) coated Thermanox coverslips in 24-well plates.

Techniques: Confocal Laser Scanning Microscopy, Control, Marker

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: MHM, U2OS and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) were treated with 10 μM CP and MG63 (p53-null) cells were treated with 5 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. The cells were then rinsed and re-fed with drug free media and cells were collected after 5 days and analyzed by flow cytometry for cell cycle. Representative cell cycle profile histograms are shown and percentage of cells with Sub-G1 DNA content are plotted. Shown are the mean results of three experiments, bars , Standard error (SE). *significance value ( P < 0.05).

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA, Flow Cytometry

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: ( A ) MHM, U2OS and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 (p53-null) cells were treated with 2.5 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. The cells were then rinsed and re-fed with drug free medium and the percentage of senescent cells (flat and SA-β-gal positive) determined after 5 days. The SA-β-gal positive cells were counted and normalized with plating efficiency. Shown are the mean results of three experiments, bars, Standard error (SE). *significance value ( P < 0.05). ( B ) MHM, U2OS and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 cells were treated with 2.5 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. The cells were then rinsed and re-fed with drug free media and colonies stained with crystal violet 2–3 weeks later. The colonies were counted and normalized with plating efficiency of untreated cells. Shown are the mean results of three experiments, bars, Standard error (SE). *significance value ( P < 0.05).

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA, Staining

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: MHM, U2OS and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 cells were untreated or treated with 10 μM CP alone or in combination with OSI-906 (5 μM) for 24 hours. Lysates were immunoblotted with antibodies against p27 and Actin.

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: MHM, U2OS and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 (p53-null) cells were transfected with control siRNA (sic) and p27 siRNA (sip27) and treated with 10 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. The cells were then rinsed and re-fed with drug free medium and cells were collected 5 days and analyzed by flow cytometry for cell cycle. Representative cell cycle profile histograms are shown and percentage of cells with Sub-G1 DNA content are plotted. Shown are the mean results of three experiments, bars , Standard error (SE). *significance value ( P < 0.05).

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA, Transfection, Flow Cytometry

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: MHM, U2OS, and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 (p53-null) cells were transfected with control siRNA (sic) and p27 siRNA (sip27) and treated with 2.5 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. Representative cell cycle profile histograms at the time of harvest are shown ( Left ) and the percentage of G1-phase cells plotted +/− SE from 3 experiments ( Right ).

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA, Transfection

Journal: Oncotarget

Article Title: Crosstalk between the IGF-1R/AKT/mTORC1 pathway and the tumor suppressors p53 and p27 determines cisplatin sensitivity and limits the effectiveness of an IGF-1R pathway inhibitor

doi: 10.18632/oncotarget.8484

Figure Lengend Snippet: MHM, U2OS, and S4 cells expressing control shRNA (shc) or p53 shRNA (shp53) and MG63 (p53-null) cells were transfected with control siRNA (sic) and p27 siRNA (sip27) and treated with 2.5 μM CP alone or in combination with OSI-906 (5 μM) for 48 hours. The cells were then rinsed and re-fed with drug free medium and colonies stained with crystal violet 2–3 weeks later. The colonies were counted and normalized with plating efficiency of untreated cells. Plotted are the mean results of three experiments, bars, Standard error (SE). *significance value ( P < 0.05).

Article Snippet: SJSA1, U2OS, MG63 osteosarcoma cells were obtained from ATCC.

Techniques: Expressing, Control, shRNA, Transfection, Staining

Journal: Scientific Reports

Article Title: Telomerase reverse transcriptase promotes chemoresistance by suppressing cisplatin-dependent apoptosis in osteosarcoma cells

doi: 10.1038/s41598-017-07204-w

Figure Lengend Snippet: TERT expression is altered and shuttles from the nucleus to mitochondria in cisplatin-treated osteosarcoma cells. ( A ) The relative mRNA expression of TERT were determined by qRT-PCR in cisplatin treated or untreated osteosarcoma cells including MG63, U2OS and 143B. ( B ) The relative protein expression of TERT were analyzed by Western-blot in cisplatin treated or untreated osteosarcoma cell lines. ( C ) Confocal images from MG63, U2OS and 143B cells untreated (control, upper panel) or treated with 5 μmol/L cisplatin for 24 h (lower panel). Green anti-TERT immune-fluorescence, red mitotracker and blue nuclear DNA (DAPI). Marked colocalization between green anti-TERT and red mitotracker is displayed by green-red mixing. ( D ) Mitochondrial fractions of cisplatin treated or untreated cells were subjected to Western blot analysis of TERT and VDAC. All experiments were carried out at least triplicates and the data were presented as the mean ± S.D. Student t test was performed to evaluate the difference *P < 0.05.

Article Snippet: The human osteosarcoma cell lines MG63, U2OS and 143B were obtained from China Centre for Type Culture Collection (CCTCC).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Fluorescence

Journal: Scientific Reports

Article Title: Telomerase reverse transcriptase promotes chemoresistance by suppressing cisplatin-dependent apoptosis in osteosarcoma cells

doi: 10.1038/s41598-017-07204-w

Figure Lengend Snippet: TERT reduces apoptosis induced by cisplatin in osteosarcoma cells independently of telomerase reverse transcriptase activity. ( A ) The relative TERT expression in mock transfected cells (control), TERT-overexpressing cells (TERT-WT, TERT-CI) and TERT-siRNA cells were assessed by Western blot analysis. ( B ) MG63 transfected cells were treated with 5 μmol/L cisplatin for 24 hours after which the apoptosis in cells was detected by flow cytometry. ( C ) U2OS transfected cells were treated wit with 5μmol/L cisplatin for 24 hours after which the apoptosis in cells was assessed by Immuno-fluorescence. Karyopyknosis is taken as apoptosis. ( D ) Quantification of apoptotic rate in cisplatin-treated osteosarcoma transfected cell lines detected by FACS. All experiments were carried out at least triplicates and the data were presented as the mean ± S.D. Student t test was performed to evaluate the difference *P < 0.05.

Article Snippet: The human osteosarcoma cell lines MG63, U2OS and 143B were obtained from China Centre for Type Culture Collection (CCTCC).

Techniques: Reverse Transcription, Activity Assay, Expressing, Transfection, Control, Western Blot, Flow Cytometry, Fluorescence